Phenolic compounds from Eucalyptus gomphocephala with potential cytotoxic and antioxidant activities

Abstract

Two new phenolic compounds, 2,4,6-trihydroxy-5-methyl-acetophenone 2-O-β-D-glucopyranoside (3), and benzyl alcohol 7-O-(3′,4′, 6′-tri-O-galloyl)-β-D-glucopyranoside (8), together with eight known phenolic compounds, were isolated from the 70% aqueous acetone extract of Eucalyptus gomphocephala DC. (Myrtaceae). The isolated compounds were elucidated based on their 1H, 13C, DQF-COSY, selective 1D-TOCSY, HSQC, and HMBC NMR spectroscopic and ESI-MS data. The antioxidant effect of the phenolic compounds was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH•), hydroxyl radical and super oxide anion radical scavenging assays. The cytotoxicity of the isolated compounds was evaluated using HeLa cell line.