O-Glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures. © Springer Science+Business Media, New York 2013.
CITATION STYLE
Blixt, O., & Cló, E. (2013). Synthesis of O-glycopeptides and construction of glycopeptide microarrays. Methods in Molecular Biology, 1047, 201–214. https://doi.org/10.1007/978-1-62703-544-6_14
Mendeley helps you to discover research relevant for your work.