Ensifer-mediated transformation (EMT) of rice (monocot) and oilseed rape (dicot)

Abstract

Ensifer adhaerens OV14 underpins the successful crop transformation protocol, termed Ensifer-mediated transformation (EMT). The adaptability and efficiency of EMT technology to successfully transform both monocot and dicots have been previously reported. To facilitate community users’ transition to EMT, the modified rice and oilseed rape plants generated in this work were developed using EMT protocols that were grounded in standard Agrobacterium-mediated transformation (AMT) processes. Therefore, this chapter describes simple yet crucial steps involved in transferring the use of EMT of rice and oilseed rape for generation of fertile and independent transgenic lines.