Comparison of Deep Eutectic Solvent-based Ultrasoundand Heat-assisted Extraction of Bioactive Compounds from Withania somnifera and Process Optimization Using Response Surface Methodology

Abstract

Extraction of bioactive compounds from Withania somnifera roots was studied using sodium acetate-glycerol deep eutectic solvent (DES) and two techniques of extraction: ultrasound-assisted extraction (UAE) and heat-assisted extraction (HAE) under response surface methodology (RSM). For UAE and HAE, total phenolic content (TPC, mg gallic acid equivalents per g dry weight (mg GAE g-1 DW)), total flavonoid content (TFC, mg rutin equivalents g-1 DW (mg RE g-1 DW)), radical scavenging activity (RSA, mg AAE (ascorbic acid equivalents) g-1 DW), and iron chelating activity (ICA, mg EDTAE (ethylenediaminetetraacetate equivalents) g-1 DW) were 6.51, 6.08, 12.56, and 3.57, respectively, and 3.33, 3.98, 6.57, and 2.48, respectively. For UAE, the optimal conditions were a DES concentration of 50%, temperature of 60°C, and time of 20 min, and for HAE, a DES concentration of 60%, temperature of 60°C, and time of 75 min. The discovered models were strongly supported by the validation experiments. UAE was more efficient and less time-consuming for extracting phytoconstituents of the W. somnifera than HAE.