The mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in vivo?; and 2) Which molecular mechanisms take place during this phenomenon? To answer both questions, we have developed a mouse model of allergic asthma using only Alt a 1 for mice sensitization. We also made use of in-vitro cellular models and computational studies to support some aspects of our hypothesis. Our results showed that Alt a 1 can induce an asthmatic phenotype, promoting tissue remodeling and infiltration of CD45+ cells, especially eosinophils and macrophages (Siglec F+ and F4/80+). Also, we have found that Alt a 1 sensitization is mediated by the TLR4-macrophage axis.
CITATION STYLE
Hernandez-Ramirez, G., Pazos-Castro, D., Gonzalez-Klein, Z., Resuela-Gonzalez, J. L., Fernandez-Bravo, S., Palacio-Garcia, L., … Diaz-Perales, A. (2022). Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.877383
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