Isolation of handelin from Chrysanthemum boreale

Abstract

The flowers of Chrysanthemum boreale afforded handelin, a unique guaianolide dimer and a mixture of n-hydrocarbons and n-hydrocarbon alcohols in addition to β-sitosterol and β-sitosterol glucoside. Detailed analysis of the 1H- and 13C-NMR spectra of handelin was carried out by the application of two-dimensional 1H- 1H-COSY and 1H- 13C multiple-bond, multiple-quantum spectroscopic correlation techniques. Handelin was inactive in the in vitro anti-tumor activity.