Kinase inhibitors represent a relatively new class of drugs that offer novel therapies targeting specific-malfunctioning kinase-mediated signaling pathways in oncology and potentially inflammation. As the ATP binding sites of the â̂500 human kinases are structurally conserved and because most current drugs target the ATP binding site, there is a need to profile all the kinases that a drug may bind and/or inhibit. We have developed a chemical proteomics method that affinity purifies kinases from cell or tissue lysates using kinase inhibitors immobilized on self-assembling monolayers. The method can be applied to assess the selectivity of a given kinase inhibitor and thus to guide its preclinical or clinical development. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Bantscheff, M., Hobson, S., & Kuster, B. (2012). Affinity purification of proteins binding to kinase inhibitors immobilized on self-assembling monolayers. Methods in Molecular Biology, 795, 149–160. https://doi.org/10.1007/978-1-61779-337-0_10
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