Isolation of mesenchymal stem cells from human dermis

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Abstract

Recent studies revealed that mammalian dermis contains multipotent stem cells such as skin-derived precursors (SKPs). SKPs grow in suspension as spheres. In contrast, mesenchymal stem cells (MSCs) are adherent fibroblastic cells. Here, we describe the procedure to isolate MSCs under low-serum culture conditions. In addition, we explain the method to collect MSCs using magnetic cell sorting. © 2013 Springer Science+Business Media New York.

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Soma, T., Kishimoto, J., & Fisher, D. (2013). Isolation of mesenchymal stem cells from human dermis. Methods in Molecular Biology, 989, 265–274. https://doi.org/10.1007/978-1-62703-330-5_20

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