Cloning and tissue-specific expression of the brain calcium channel β-subunit

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Abstract

A cDNA clone encoding a protein with high homology to the β-subunit of the rabbit skeletal muscle dihydropyridine-sensitive calcium channel was isolated from a rat brain cDNA library. This rat brain β-subunit cDNA hybridizes to a 3.4 kb message that is expressed in high levels in the cerebral hemispheres and hippocampus but is significantly reduced in cerebellum. The open reading frame encodes 597 amino acids with a predicted mass of 65 679 Da which is 82% homologous with the skeletal muscle β-subunit. The brain cDNA encodes a unique 153 amino acid C-terminus and predicts the absence of a muscle-specific 50 amino acid internal segment. It also encodes numerous consensus phosphorylation sites suggesting a role in calcium channel regulation. The corresponding human β-subunit gene was localized to chromosome 17. Hence the encoded brain β-Subunit. which has a primary structure highly similar to its isoform in skeletal muscle, may have a comparable role as an integral regulatory component of a neuronal calcium channel. © 1991.

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Pragnell, M., Sakamoto, J., Jay, S. D., & Campbell, K. P. (1991). Cloning and tissue-specific expression of the brain calcium channel β-subunit. FEBS Letters, 291(2), 253–258. https://doi.org/10.1016/0014-5793(91)81296-K

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