Construction of transcription factor gene deletion library of aspergillus luchuensis

Abstract

We selected 96 genes of Aspergillus luchuensis for the construction of a transcription factor gene deletion library. Of these, we successfully deleted 93 genes using Agrobacterium-mediated transforma-tion (AMT) of A. luchuensis RIB 2604 ∆ligD strains. We obtained only heterokaryonic strains after de-letions of adaB, anBH1, hacA, hapB, hsf1, metR, and sonC gene, and additionally, could not obtain deletion strains for genes abaA and mcmA. The deletion strains will be available through our website (https://www.nrib.go.jp).