The antiviral activity of native and esterified whey proteins fractions (α-lactalbumin, β-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (TYLCV) on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using α-lactoferrin and α-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native β-lactoglobulin showed inhibition after 7 and 15 days whereas esterified -lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified β-lactalbumin whereas native α-lactalbumin inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants. © 2010 Abdelbacki et al; licensee BioMed Central Ltd.
CITATION STYLE
Abdelbacki, A. M., Taha, S. H., Sitohy, M. Z., Dawood, A. I. A., Abdel-Hamid, M., & Rezk, A. A. (2010, January 1). Inhibition of tomato yellow leaf curl virus (TYLCV) using whey proteins. Virology Journal. BioMed Central Ltd. https://doi.org/10.1186/1743-422X-7-26
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