Zinc finger nucleases (ZFNs) are proteins engineered to make site-specific double-strand breaks (DSBs) in a DNA sequence of interest. Imprecise repair of the ZFN-induced DSBs by the nonhomologous end-joining (NHEJ) pathway results in a spectrum of mutations, such as nucleotide substitutions, insertions, and deletions. Here we describe a method for targeted mutagenesis in Arabidopsis with ZFNs, which are engineered by context-dependent assembly (CoDA). This ZFN-induced mutagenesis method is an alternative to other currently available gene knockout or knockdown technologies and is useful for reverse genetic studies. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Qi, Y., Starker, C. G., Zhang, F., Baltes, N. J., & Voytas, D. F. (2014). Tailor-made mutations in arabidopsis using zinc finger nucleases. Methods in Molecular Biology, 1062, 193–209. https://doi.org/10.1007/978-1-62703-580-4_10
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