With the availability of increasing numbers of fluorescent protein variants and state-of-the-art imaging techniques, live cell microscopy has become a standard procedure in modern cell biology. Fluorescent markers are used to visualize the dynamic processes that take place in living cells, including the behavior of membrane-bound organelles. Here, we provide two examples of how we analyze the membrane dynamics of mitochondria in living yeast cells using wide field and confocal microscopy: (1) Long-term observation of mitochondrial shape changes using mitochondria-targeted fluorescent proteins and (2) monitoring the behavior of individual mitochondria using a mitochondria-targeted version of a photoconvertible fluorescent protein. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Scholz, D., Förtsch, J., Böckler, S., Klecker, T., & Westermann, B. (2013). Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria. Methods in Molecular Biology, 1033, 275–283. https://doi.org/10.1007/978-1-62703-487-6_17
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