Ras antagonizes cAMP stimulated glucagon gene transcription in pancreatic islet cell lines

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Abstract

Ras, a GTP-binding protein, converts membrane tyrosine kinase signalling to changes in gene expression patterns. Utilising a rat glucagon promoter-CAT construct (p[-1.1]GLU-CAT) we demonstrate in transient transfection experiments that the oncogenic Ras inhibits cAMP-dependent activation of p[-1.1]GLU-CAT in both glucagonoma InR1-G9 and insulinoma β-TC1 cells. Conversely, the expression of a dominant negative mutant of Ras enhances the cAMP-induced activation of p[-1.1]GLU-CAT transcription in these cells. Our data suggests a functional interference of Ras with the cAMP-dependent transcription of the glucagon gene. © 1994.

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Gherzi, R., Briata, P., Fehmann, H. C., & Göke, B. (1994). Ras antagonizes cAMP stimulated glucagon gene transcription in pancreatic islet cell lines. FEBS Letters, 353(3), 277–280. https://doi.org/10.1016/0014-5793(94)01050-1

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