RNA tagging: Preparation of high-throughput sequencing libraries

Abstract

Protein–RNA networks, in which a single protein binds and controls multiple mRNAs, are central in biological control. As a result, methods to identify protein–RNA interactions that occur in vivo are valuable. The “RNA Tagging” approach enables the investigator to unambiguously identify global protein–RNA interactions in vivo and is independent of protein purification, cross-linking, and radioactive labeling steps. Here, we provide a protocol to prepare high-throughput sequencing libraries for RNA Tagging experiments.