Receptor binding provides a valuable approach for characterization of drugs and their receptors. There are three major families of opioid receptors: mu, delta, and kappa. Highly selective radioligands are available for all three classes of traditional receptors. Of the three, the mu receptor undergoes extensive alternative splicing, generating a number of traditional mu receptor subtypes as well as a nontraditional, truncated set of variants associated with exon 11. These exon 11-associated truncated variants are not readily labeled with current radioligands. Here we describe the synthesis of a radioiodinated ligand suitable for carrying out binding studies for this target.
CITATION STYLE
Marrone, G. F., Majumdar, S., & Pasternak, G. W. (2015). Radioligand binding assay for an exon 11-associated mu opioid receptor target. Methods in Molecular Biology, 1335, 241–249. https://doi.org/10.1007/978-1-4939-2914-6_16
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