An overview is presented of structure/function relationships in the interactions between the small electron transfer proteins ferredoxin (Fd) and flavodoxin (Fld) and the flavoprotein enzyme ferredoxin:NADP+ reductase (FNR), primarily emphasizing the proteins from the cyanobacterium, Anabaena, and the higher plant, spinach. Results are summarized from experiments utilizing rapid-reaction kinetic methods (stopped-flow spectrophotometry and laser flash photolysis) involving wild-type and site-specific mutants of these proteins, redox potential determinations, and X-ray crystallography, including the crystal structure of a Fd/FNR complex. These have provided detailed insights into the protein–protein recognition and electron transfer mechanisms utilized by these systems. Fd and Fld bind to FNR within a concave region of the FNR surface that contains the exposed dimethylbenzene ring of the FAD cofactor. In the Fd case, electron transfer between the iron–sulfur and flavin centers proceeds with a maximum rate constant of 5,500 sec−1 via a direct outer-sphere mechanism. Both electrostatic and hydrophobic interactions occur between the proteins, resulting in a precise surface complementarity. I.
CITATION STYLE
Hurley, J. K., Tollin, G., Medina, M., & Gómez-Moreno, C. (2007). Electron Transfer From Ferredoxin and Flavodoxin to Ferredoxin:NADP+ Reductase. In Photosystem I (pp. 455–476). Springer Netherlands. https://doi.org/10.1007/978-1-4020-4256-0_27
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