Transcriptomics enables us to elucidate comprehensive gene expression profiles in given experimental conditions. Global regulators, which include transcriptional regulators and two-component regulatory systems, have evolved in a variety of bacterial systems. They play important roles in bacterial fitness and pathogenesis by regulating target gene expression. Advanced next-generation RNA sequencing technology (RNA-seq) provides a powerful and effective tool to analyze the transcriptome of bacterial cells. In this chapter, we provide a detailed procedure for the investigation of gene expression profiles and identification of target genes, regulons, and/or pathways that are mediated by a regulator. This procedure is done using RNA-seq analysis, which involves RNA purification, mRNA enrichment, decontamination, RNA-seq data analysis, and quantitative real-time reverse transcription PCR.
CITATION STYLE
Lei, T., Yang, J., Becker, A., & Ji, Y. (2020). Identification of target genes mediated by two-component regulators of staphylococcus aureus using RNA-seq technology. In Methods in Molecular Biology (Vol. 2069, pp. 125–138). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9849-4_10
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