Autosomal, sex and b chromosomes in eyprepocnemis plorans (Orthoptera) viewed with restriction endonuclease in situ digestion

Abstract

Similarities and differences between different subsets of heterochromatin localized on the autosomes, sex chromosomes and a B-chromosome in the genome of the grasshopper Eyprepocnemis plorans were analysed by in-situ digestion with 23 different restriction endonucleases, three unspecific nucleases, C-banding and specific fluorescent DNA-ligands. Results show an equilocal distribution of the different heterochromatic subsets in this species and provide a possible evolutionary origin for the B chromosome. © 1992 The Genetical Society of Great Britain.