Moving past the discovery phase of proteomics, the term targeted proteomics combines multiple approaches investigating a certain set of proteins in more detail. One such targeted proteomics approach is the combination of liquid chromatography and selected or multiple reaction monitoring mass spectrometry (SRM, MRM). SRM-MS requires prior knowledge of the fragmentation pattern of peptides, as the presence of the analyte in a sample is determined by measuring the m/z values of predefined precursor and fragment ions. Using scheduled SRM-MS, many analytes can robustly be monitored allowing for high-throughput sample analysis of the same set of proteins over many conditions. In this chapter, fundaments of SRM-MS are explained as well as an optimized SRM pipeline from assay generation to data analyzed. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Ebhardt, H. A. (2014). Selected reaction monitoring mass spectrometry: A methodology overview. Methods in Molecular Biology, 1072, 209–222. https://doi.org/10.1007/978-1-62703-631-3_16
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