Mitochondrial Ca2+ homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca2+. Ca2+ concentration in mitochondria ([Ca2+]M) is low at rest (about 10−7 M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca2+ uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca2+ transport.
CITATION STYLE
Alonso, M. T., Navas-Navarro, P., & García-Sancho, J. (2017). A microplate-based bioluminescence assay of mitochondrial calcium uptake. In Methods in Molecular Biology (Vol. 1567, pp. 245–253). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6824-4_15
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