Sputum induction as a research tool for sampling the airways of subjects with cystic fibrosis

107Citations
Citations of this article
49Readers
Mendeley users who have this article in their library.

Abstract

Background - Sputum induction (SI) has proved to be a reliable non-invasive tool for sampling inflammatory airway contents in asthma, with distinct advantages over collection of expectorated sputum (ES) and bronchoalveolar lavage (BAL). A study was undertaken to evaluate the safety of SI and to assess if it might be an equally valuable outcome tool in patients with cystic fibrosis (CF). Methods - The safety of the procedure was examined and sample volume, cell counts, cytokine concentrations, and bacterial culture results obtained by SI, spontaneous ES, and fibreoptic bronchoscopy were compared in 10 adults with CF. Results - SI was well tolerated and was preferred to BAL by all subjects. The mean (SE) sample volume obtained by SI was significantly greater than ES (6.74 (1.46) ml υ 1.85 (0.33) ml, p = 0.005). There was no significant difference in the number of cells per ml of sample collected. There was a difference in the mean (SD) percentage of non-epithelial, nonsquamous cells collected (67 (28)%, 86 (21)%, and 99 (1)% for ES, SI, and BAL, respectively). These percentage counts were different between ES and both SI and BAL (p=0.03 and p=0.006, respectively). Cell differential counts (excluding squamous cells) from all collection methods were similar (mean (SD) 84 (9)%, 87 (7)%, and 88 (11)% polymorphonuclear cells for ES, SI, and BAL, respectively). The concentrations of interleukin (IL)-8 and tumour necrosis factor (TNF)-α were the same in all three samples when corrected for dilution using urea concentration. The test specific detection rate for recovery of bacteriological pathogens was 79% for SI, 76% for ES, and 73% for BAL. Conclusion - SI offers safety advantages over BAL and may be a more representative airway outcome measurement in patients with CF.

References Powered by Scopus

Standardization of spirometry--1987 update. Statement of the American Thoracic Society.

0
2333Citations
N/AReaders
Get full text

Indices of airway inflammation in induced sputum: Reproducibility and validity of cell and fluid-phase measurements

865Citations
N/AReaders
Get full text

Use of induced sputum cell counts to investigate airway inflammation in asthma

843Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Pathophysiology and Management of Pulmonary Infections in Cystic Fibrosis

1388Citations
N/AReaders
Get full text

Exacerbations in cystic fibrosis·1: Epidemiology and pathogenesis

370Citations
N/AReaders
Get full text

Non-cystic fibrosis bronchiectasis

316Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Henig, N. R., Tonelli, M. R., Pier, M. V., Burns, J. L., & Aitken, M. L. (2001). Sputum induction as a research tool for sampling the airways of subjects with cystic fibrosis. Thorax, 56(4), 306–311. https://doi.org/10.1136/thorax.56.4.306

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 23

61%

Professor / Associate Prof. 7

18%

Researcher 7

18%

Lecturer / Post doc 1

3%

Readers' Discipline

Tooltip

Medicine and Dentistry 21

57%

Agricultural and Biological Sciences 8

22%

Biochemistry, Genetics and Molecular Bi... 6

16%

Immunology and Microbiology 2

5%

Save time finding and organizing research with Mendeley

Sign up for free