Assessing the aflatoxin B1 adsorption capacity between biosorbents using an in vitro multicompartmental model simulating the dynamic conditions in the gastrointestinal tract of poultry

Abstract

Experiments were carried out to evaluate the effectiveness of three different biosorbents (banana peel, Pyracantha leaves, and Aloe powder) in removing aflatoxin B1 (AFB1). A noncommercial mycotoxin binder (zeolite) was used as a reference material. A laboratory model that simulated the in vivo conditions of the poultry gastrointestinal tract was utilized to prove the removal efficiency of the biosorbents when added to AFB1-contaminated diet (100 µg/kg). The concentration of AFB1 was determined using antibody-based immunoaffinity column and spectrofluorometry methodologies. Z potential (ζ), point of zero charge (pHpzc), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR), and UV-Vis diffuse reflectance spectroscopy (DRS) techniques were used to further characterize the biosorbents. The addition of the biosorbents (1.5%, w/w) to the diet significantly reduced the bioavailability of AFB1 in the intestinal section. The highest aflatoxin adsorption values were 69% and 70% using Aloe powder and zeolite, respectively. A moderate biosorption uptake of 46% was achieved using Pyracantha leaves. The biomaterial with the lowest removal capacity was banana peel (28%). In conclusion, Aloe powder could be used as an alternative to conventional systems for AFB1 removal.