Auto-focusing and quantitative phase imaging using deep learning for the incoherent illumination microscopy system

  • Ding H
  • Li F
  • Meng Z
  • et al.
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Abstract

It is well known that the quantitative phase information which is vital in the biomedical study is hard to be directly obtained with bright-field microscopy under incoherent illumination. In addition, it is impossible to maintain the living sample in focus over long-term observation. Therefore, both the autofocusing and quantitative phase imaging techniques have to be solved in microscopy simultaneously. Here, we propose a lightweight deep learning-based framework, which is constructed by residual structure and is constrained by a novel loss function model, to realize both autofocusing and quantitative phase imaging. It outputs the corresponding in-focus amplitude and phase information at high speed (10fps) from a single-shot out-of-focus bright-field image. The training data were captured with a designed system under a hybrid incoherent and coherent illumination system. The experimental results verify that the focused and quantitative phase images of non-biological samples and biological samples can be reconstructed by using the framework. It provides a versatile quantitative technique for continuous monitoring of living cells in long-term and label-free imaging by using a traditional incoherent illumination microscopy system.

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Ding, H., Li, F., Meng, Z., Feng, S., Ma, J., Nie, S., & Yuan, C. (2021). Auto-focusing and quantitative phase imaging using deep learning for the incoherent illumination microscopy system. Optics Express, 29(17), 26385. https://doi.org/10.1364/oe.434014

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