Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells.
CITATION STYLE
Da Silveira, J., Andrade, G. M., Perecin, F., Meireles, F. V., Winger, Q. A., & Bouma, G. J. (2018). Isolation and analysis of exosomal MicroRNAs from ovarian follicular fluid. In Methods in Molecular Biology (Vol. 1733, pp. 53–63). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7601-0_4
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