Fluorescence detection system

Abstract

One of the most exciting advances in contemporary analytical ultracentrifugation has been the development of the analytical ultracentrifuge equipped with a fluorescence detection system (AU-FDS). The AU-FDS provides both selectivity and sensitivity thereby enabling the measurement of specific fluorescently labeled macromolecules in complex solutions and/or the detection of dilute samples at low (sub-nanomolar) concentrations. This has had tremendous impact to the study of low-abundance proteins, the quantification of high-affinity interactions (KD