In the present study, we clarified that transforming growth factor beta (TGF-beta) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-beta-induced cellular senescence. Among PKCs, we showed that PKC-delta induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-delta in cellular senescence programs was demonstrated using a kinase negative PKC-delta and small interfering RNA against PKC-delta. Furthermore, PKC-delta was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-delta plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-delta.
CITATION STYLE
Cormier, A. A., & Buikstra, J. E. (2017). Impairment, Disability, and Identity in the Middle Woodland Period: Life at the Juncture of Achondroplasia, Pregnancy, and Infection. In Bioarchaeology of Impairment and Disability (pp. 225–248). Springer International Publishing. https://doi.org/10.1007/978-3-319-56949-9_12
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