Tissue heterogeneity is both a major confounding factor and an underexploited information source. While a handful of reports have demonstrated the potential of supervised methods to deconvolve tissue heterogeneity, these approaches require a priori information on the marker genes or composition of known subpopulations. To address the critical problem of the absence of validated marker genes for many (including novel) subpopulations, we develop a novel unsupervised deconvolution method, Convex Analysis of Mixtures (CAM), within a well-grounded mathematical framework, to dissect mixed gene expressions in heterogeneous tissue samples. To facilitate the utility of this method, we implement an R-Java CAM package that provides comprehensive analytic functions and graphic user interface (GUI).
CITATION STYLE
Wang, N., Chen, L., & Wang, Y. (2018). Mathematical modeling and deconvolution of molecular heterogeneity identifies novel subpopulations in complex tissues. In Methods in Molecular Biology (Vol. 1751, pp. 223–236). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7710-9_16
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