Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) in Vitro

Abstract

Ethanol (95%) and dichloromethane: Methanol (DCM: M, 1: 1 v/v) bark extracts (BEs) and leaf extracts (LEs) of authenticated Ceylon cinnamon (CC) were studied for antiamylase, antiglucosidase, anticholinesterases, and antiglycation and glycation reversing potential in bovine serum albumin-(BSA-) glucose and BSA-methylglyoxal models in vitro. Further, total proanthocyanidins (TP) were quantified. Results showed significant differences (p<0.05) between bark and leaf extracts for the studied biological activities (except antiglucosidase) and TP. BEs showed significantly high (p<0.05) activities for antiamylase (IC50: 214±2-215±10 μg/mL), antibutyrylcholinesterase (IC50: 26.62±1.66-36.09±0.83 μg/mL), and glycation reversing in BSA-glucose model (EC50: 94.33±1.81-107.16±3.95 μg/mL) compared to LEs. In contrast, glycation reversing in BSA-methylglyoxal (EC50: Ethanol: 122.15±6.01 μg/mL) and antiglycation in both BSA-glucose (IC50: Ethanol: 15.22±0.47 μg/mL) and BSA-methylglyoxal models (IC50: DCM: M: 278.29±8.55 μg/mL) were significantly high (p<0.05) in leaf. Compared to the reference drugs used some of the biological activities were significantly (p<0.05) high (BEs: BChE inhibition and ethanol leaf: BSA-glucose mediated antiglycation), some were comparable (BEs: BSA-glucose mediated antiglycation), and some were moderate (BEs and LEs: Antiamylase, AChE inhibition, and BSA-MGO mediated antiglycation; DCM: M leaf: BSA-glucose mediated antiglycation). TP were significantly high (p<0.05) in BEs compared to LEs (BEs and LEs: 1097.90±73.01-1381.53±45.93 and 309.52±2.81-434.24±14.12 mg cyanidin equivalents/g extract, resp.). In conclusion, both bark and leaf of CC possess antidiabetic properties and thus may be useful in managing diabetes and its complications.