Identification of (+)-phyllocladene, (-)-sandaracopimaradiene, and (+)-Kaurene as new fungal metabolites from fusicoccin-producing Phomopsis amygdali F6

Abstract

A chemical analysis of the diterpene hydrocarbons produced by fusicoccin-producing fungus Phomopsis amygdali F6 identified five phyllocladene-related triand tetracyclic diterpene hydrocarbons. The presence of (+)-phyllocladene, (-)-sandaracopimaradiene, (+)-isopimara-8,15-diene, and (+)-pimara-8(14),15-diene in the fungus was demonstrated by GC-MS, 1H-NMR, and [α]D measurements. (+)-Kaurene was also identified by GC-MS and chiral capillary GC. The possible biosynthetic relationship of these metabolites is discussed.