Photoinhibition of photosystem I at chilling temperature and subsequent recovery in Arabidopsis thaliana

Abstract

Chilling-induced photoinhibition and subsequent recovery was studied in Arabidopsis thallana exposed to 4°C and 150 μmol photons m-2 s-1. PSII showed progressive damage with a 14% decrease in quantum yield after 8 h exposure. In contrast, the damage to PSI leveled off after 8 h with a decrease in in vitro NADP+ photoreduction activity of around 32%. In vivo P700 measurements demonstrated that antenna efficiency was decreased by the photoinhibitory treatment. Measurements of P700 and immunoblotting demonstrated that the damaged PSI was not degraded during the 8 h light-chilling treatment, but after 12 h recovery at 20°C, no damaged PSI remained in the thylakoids. Thus, degradation of damaged PSI is a step in the recovery and not a direct result of photodamage. Unlike photodamaged PSII, the PSI core complex is not repaired but completely degraded. In contrast, light harvesting complex I proteins have a slow turnover. PSII recovered completely within 8 h after transfer to 20°C whereas PSI activity recovered very slowly, and the amount of PSI on a leaf area basis remained low even after 1 week at 20°C. The results show that damage, protein turnover and recovery are well separated processes in Arabidopsis.