Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens

Abstract

A whole-bacterial cell enzyme-linked immunosorbent assay (bactELISA) was developed for detecting fimbrial antigens on Streptococcus sanguis. In this assay, S. sanguis cells were directly adhered to poystyrene or polyvinyl via drying. Use of the assay indicated that consistently high and uniform optical densities could be obtained from well to well. In addition, radioactive assaying indicated increased adsorbance to the polystyrene wells over polyvinyl, suggesting that polystyrene may prove superior in the gram-positive bactELISA. Use of the bactELISA may prove valuable to both the clinical and research laboratory involved in the study of bacterial cell surface components or in the evaluation of antisera directed against bacterial antigens, which are difficult to prepare as purified derivatives.