In-vitro and in-vivo anti-inflammatory activity of Plumeria indica Linn flowers

Abstract

Inflammation is a reaction of a living vascularised tissue to an injury. Conventional or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it sometimes have serious side effects. So, number of herbal medicines is recommended for the treatment of inflammation that has no side effects. Hence our study focused to investigate the physicochemical, qualitative phytochemical analysis of bioactive compounds and In-vitro and In-vivo anti-inflammatory activity of Plumeria indica Linn (P. Indica) flowers extract which has boundless medicinal properties. The physicochemical evaluations carried out in terms of loss on drying, ash value, extractive values and acid insoluble ash value ect. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The aqueous and ethanolic extract of P. Indica flowers was screened for in-vivo anti-inflammatory activity by carrageenan induced paw edema in rat model and in-vitro anti-inflammatory activity by human red blood cell membrane stabilization method. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids ect. Ethanolic extract showed best in vitro anti-inflammatory activity was screened for in vivo anti-inflammatory activity at the dose level of 250 and 500mg/kg. Indomethacin at the dose level of 10 mg/kg was used as reference standard drug. Both the extracts showed a dose dependent anti-inflammatory potential which provide scientific basis for the traditional claims of P. Indica flowers as an anti-inflammatory drug. Keywords: Plumeria indica Linn, Anti-inflammatory activity, Carrageenan, Human red blood cells membrane