Effect of nitrogen application rate on the enzyme activity of flag leaf after anthesis in winter wheat

Abstract

This experiment studied the effect of nitrogen application rate on the enzyme activity of flag leaf after anthesis in winter wheat whose results showed that when the nitrogen application was within the range of 0~300 kg/hm2, with the increased amount of nitrogen, soluble protein content, the activity of Nitrate Reductase (NR) and the Glutamine Synthetase (GS) increased except for the leaf amino peptidase (As) activity. The soluble protein content, NR and GS activity of T2 (225 kg/hm2) and T3 (300 kg/hm2) were higher than other treatments, but decreased in the 21 days after a thesis. However, considering the cost of nitrogen fertilizer and the impact of nitrogen fertilizer on the environment, T2 was the most appropriate which could maintain high activity and stability during the entire growth period of the flag leaf and delayed the flag leaf senescence effectively. © Maxwell Scientific Organization, 2013.