Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Nakamura, R., & Teshima, R. (2014). Immunoproteomics analysis of food allergens. Methods in Molecular Biology, 1072, 725–735. https://doi.org/10.1007/978-1-62703-631-3_50
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