In vitro biocompatibility tests of glass ionomer cements impregnated with collagen or bioactive glass to fibroblasts

8Citations
Citations of this article
58Readers
Mendeley users who have this article in their library.

Abstract

Aim and Design: To evaluate the biocompatibility of glass ionomer cement (GIC) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro. Mineral Trioxide Aggregate was used as the standard for comparison. Human maxillary central incisors (n=70) were instrumented with a rotary NiTi system and filled. Following resection of the apical 3mm, root end cavities were prepared and restored with conventional GIC (group 1) or GIC with 0.01%, 0.1% or 1% collagen (groups 2, 3, 4 respectively) or, 10%, 30 % or 50% bioactive glass (groups 5,6,7 respectively), or Mineral Trioxide Aggregate (group 8). The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line. Phase contrast and scanning electron microscopes were used to score cell quantity, morphology and cell attachment. The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test (p = 0.05). Results and conclusions: Group 5 showed the highest scores which was significantly higher than all other groups (p<0.05) except group 8, with which there was no significant difference (p>0.05). Glass ionomer cement with 10% bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01% collagen. The biocompatibility of collagen and bioactive glass was concentration dependent. The addition of bio active glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen.

Cite

CITATION STYLE

APA

Subbarao, C., Neelakantan, P., & Subbarao, C. V. (2012). In vitro biocompatibility tests of glass ionomer cements impregnated with collagen or bioactive glass to fibroblasts. Journal of Clinical Pediatric Dentistry, 36(3), 269–274. https://doi.org/10.17796/jcpd.36.3.gk80547w04504144

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free