Identificação de flavonóides em Hypericum cordatum (Vell.) N. Robson (Clusiaceae)

Abstract

The aim of this work was the fractionation of methanolic extracts from stems and leaves of Hypericum cordatum and the identification of flavonoids. The fractionation of methanolic extracts was done using filtration columm in Sephadex LH 20 resin, thin layer and high performance liquid chromatography techniques. Several flavonoids were found in the methanolic extracts, specially flavonols and flavones. Four flavonoids were identified: quercetin, quercitrin, rutin and kaempferol. The identification of these compounds was done by the analyses of the ultraviolet light spectrum obtained in HPLC-UV/DAD and by co-chromatography with standard solutions.