pH-metric studies and electrochemical behavior of Eu(III)-7-carboxymethoxy-4-methylcoumarin with 5'-GMP

Abstract

The interaction of Eu(III)-7-carboxymethoxy-4-methylcoumarin(CMMC) with guanosine-5'-momophosphate (5'-GMP) has been investigated using electron analytical, fluorescence, and potentiometric methods in the ethanol-water mixture solvent (0.15 volume fraction). The formation of the different binary, ternary, and quaternary complexes is confirmed by the corresponding pH-potentiometric curves. SUPERQUAD computer program has been used for the refinement of all the calculated constants in our present study. Electroanalytical techniques have been used to confirm the formation of different binary and ternary complexes under investigation. The binding constant of the ternary complex Eu(III)-CMMC-5'-GMP calculated by fluorescence technique was found to be 1.8 x 107 with 1:1 molar ratio. DNA cleavage properties of the CMMC, gibberellic acid, and their europium complexes were tested by gel electrophoresis.