Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway ®-Compatible Binary Potato Virus X-Expression Vector

Abstract

Identification of pathogen effectors that elicit a hypersensitive response (HR) in resistant plant hosts is essential to study disease resistance. In this method, it is described how to generate a cDNA library, how to transfer the library into a binary PVX-expression vector, and finally how to set up a high-throughput screen for HR-inducing cDNAs from plant pathogens. © 2012 Springer Science+Business Media, LLC.