A hydrogen peroxide sensor prepared by electropolymerization of pyrrole based on screen-printed carbon paste electrodes

Abstract

A disposable amperometric biosensor for commercial use to detect hydrogen peroxide has been developed. The sensor is based on screen-printed carbon paste electrodes modified by electropolymerization of pyrrole with horseradish peroxidase (HRP) entrapped. The facture techniques of fabricating the enzyme electrodes are suitable for mass production and quality control. The biosensor shows a linear amperometric response to H2O2 from 0.1 to 2.0 mM, with a sensitivity of 33.24 A mM-1 cm-2. Different operational parameters of electropolymerization are evaluated and optimized. © 2007 by MDPI.