G.M. Rothe: Electrophoresis of Enzymes. Laboratory Methods

Abstract

The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis. From the contents: Extraction of Enzymes from Tissues, Cells, and Cell-Organelles – Methods for Separating Native Enzymes (Electrophoresis in Cellulose Acetate, Starch, Polyacrylamide, Non-denaturing 2D-Electrophoresis) – Sodium Dodecyclsulphate Electrophoresis – Chemistry of Enzyme Visualization – A Compilation of Protocols to Visualize Enzymes – Data Evaluation in Population Genetics and Evolution.