Capillary electrophoresis (CE) is one among a number of highly sensitive chemical separation techniques used to characterize single or a small number of cells and to develop assays of enzymatic activity. Other commonly used techniques include mass spectrometry and electrochemistry; however, CE using laserinduced fluorescence detection (LIF) is the most sensitive of these techniques. In CE-LIF, fluorescently labeled proteins or lipids are normally separated based on their size to charge ratio in the interior of a small capillary filled with an electrolyte upon the application of an electric field. In this chapter, we describe the application of CE-LIF for the determination of the bioactivity of fluorescently labeled lipids and sphingosine kinase activity. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Yangyuoru, P. M., Hammonds-Odie, L., & Mwongela, S. M. (2013). Fluorescent lipids as probes for sphingosine kinase activity by capillary electrophoresis. Methods in Molecular Biology, 984, 329–340. https://doi.org/10.1007/978-1-62703-296-4_24
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