Green synthesis of silver nanoparticles using green coffee bean extract

Abstract

This investigation report a simple green synthesis for stabilizing silver nanoparticles (AgNPs) using green coffee beam extract. Silver nitrate was used as the metal precursor and green coffee extract as a reducing agent. The AgNPs UV-Vis spectrum was recorded, the morphology of AgNPs was determinate by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Antibacterial activity of the synthetized nanoparticles was analyzed by means well diffusion method against three different microorganisms (Escherichia coli ATCC 25922, Pseudomonas aureginosa ATCC 27853 and Staphylococcus aureus ATCC 25923). The silver nanoparticles showed high stability and antimicrobial activity against both gran positive and gram negative bacteria.