Kinetics and structural features of dimeric glutamine-dependent bacterial NAD synthetases suggest evolutionary adaptation to available metabolites

Abstract

NADH (NAD) and its reduced form NADH serve as cofac-tors for a variety of oxidoreductases that participate in many metabolic pathways. NAD also is used as substrate by ADP-ribosyl transferases and by sirtuins. NAD biosynthesis is one of the most fundamental biochemical pathways in nature, and the ubiquitous NAD synthetase (NadE) catalyzes the final step in this biosynthetic route. Two different classes of NadE have been described to date: dimeric single-domain ammonium-dependent NadENH3 and octameric glutamine-dependent NadEGln, and the presence of multiple NadE isoforms is relatively common in prokaryotes. Here, we identified a novel dimeric group of NadEGln in bacteria. Substrate preferences and structural anal-yses suggested that dimeric NadEGln enzymes may constitute evolutionary intermediates between dimeric NadENH3 and octameric NadEGln. The characterization of additional NadE isoforms in the diazotrophic bacterium Azospirillum brasilense along with the determination of intracellular glutamine levels in response to an ammonium shock led us to propose a model in which these different NadE isoforms became active accordingly to the availability of nitrogen. These data may explain the selective pressures that support the coexistence of multiple isoforms of NadE in some prokaryotes.