We have utilized differential display polymerase chain reaction to investigate the gene expression of hematopoietic progenitor cells from adult bone marrow and umbilical cord blood. A differentially expressed gene was identified in CD34+ hematopoietic progenitor cells, with low expression in CD34- cells. We have obtained the full coding sequence of this gene which we designated human mammalian ependymin-related protein 1 (MERP1). Expression of MERP1 was found in a variety of normal human tissues, and is 4- and 10-fold higher in adult bone marrow and umbilical cord blood CD34+ cells, respectively, compared to CD34- cells. Additionally, MERP1 expression in a hematopoietic stem cell enriched population was down-regulated with proliferation and differentiation. Conceptual translation of the MERP1 open reading frame reveals significant homology to two families of glycoprotein calcium-dependant cell adhesion molecules: ependymins and protocadherins. © 2002 Elsevier Science B.V. All rights reserved.
CITATION STYLE
Gregorio-King, C. C., McLeod, J. L., Collier, F. M., Collier, G. R., Bolton, K. A., Van Der Meer, G. J., … Kirkland, M. A. (2002). MERP1: A mammalian ependymin-related protein gene differentially expressed in hematopoietic cells. Gene, 286(2), 249–257. https://doi.org/10.1016/S0378-1119(02)00434-1
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