Arsenic is a well-known human carcinogen. However, the mechanisms underlying arsenic-induced carcinogenesis remain elusive. Here we show that chronic and low level of arsenic stress induces transformation of the human bronchial epithelial cells, BEAS-2B, and that some of the transformed cells show characteristics of cancer stem-like cells (CSCs). Meanwhile, we demonstrate that arsenic stress dedifferentiates CD61+ BEAS-2B cells into CSC-like CD61− cells featured with noncanonical epithelial–mesenchymal transition (EMT), enhanced chemoresistance, and metastasis. Finally, we show that oncogene c-Myc expression is associated with arsenic-induced tumor initiation and progression. Altogether, our findings highlight a unique mechanism of arsenic-induced transformation of human bronchial epithelial cells and provide a novel therapeutic target for arsenic-initiated lung cancer.
CITATION STYLE
Chang, Q., Bi, Z., Fu, Y., Rice, M. K. A., Zhang, Q., Wadgaonkar, P., … Chen, F. (2020). Characterization of arsenic-induced cancer stem-like cells. In Methods in Molecular Biology (Vol. 2117, pp. 293–303). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0301-7_19
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