Impact of growth in benzoate and m-toluate liquid media on culturability of Pseudomonas putida on benzoate and m-toluate plates

Abstract

Pseudomonas putida grown in continuous culture on benzoate or m-toluate lost the ability to grow on benzoate or m-toluate plates. A similar effect was not seen with a glucose continuous culture. Cells carrying and expressing a TOL plasmid rapidly lost their ability to grow on benzoate solid medium.