Characterization of diet-dependent metabolic serotypes: Primary validation of male and female serotypes in independent cohorts of rats

38Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Our research seeks to identify serum profiles, or serotypes, that reflect substantial changes in food intake in both male and female rats. This report validates previously defined subsets of redox-active low-molecular-weight metabolites using independent cohorts of ad libitum consumption (AL) and energy or dietary restricted (DR) 6-mo-old male and female rats. In the male study, both hierarchical cluster analysis (HCA) and principal component analysis (PCA) distinguished the dietary groups of origin in the second male cohort with >85% accuracy using 56 analytically and biologically valid metabolites. Further analysis revealed that 29 metabolites (nine previously unidentified metabolites + 20 chosen from the 56 metabolites) enabled HCA to distinguish dietary groups at 100% efficacy. In the female study, the 63 previously identified serum metabolites were sufficiently robust to enable classification of the dietary intake of two female cohorts (cohorts 2 and 3) that were independent of the cohort on which these markers were initially identified (cohort 1). Classification accuracy was 94 and 100% using HCA and PCA, respectively, in the female cohort 2. HCA and PCA revealed that the 63-metabolite profile distinguished AL and DR samples at 91 and 100% accuracy in the female cohort 3, establishing the 63-metabolite dataset as our baseline profile. These studies used independent cohorts to validate and potentially improve upon previously defined metabolic serotype in male and female rats and set the stage for pattern recognition-based approaches to establish metabolome-based categorical separations.

Figures

References Powered by Scopus

Guidelines for healthy weight

1028Citations
N/AReaders
Get full text

Dietary restriction in mice beginning at 1 year of age: Effect on life-span and spontaneous cancer incidence

636Citations
N/AReaders
Get full text

Aging and oxidative stress: Modulation by dietary restriction

376Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research

466Citations
N/AReaders
Get full text

NMR-based metabonomic approaches for evaluating physiological influences on biofluid composition

428Citations
N/AReaders
Get full text

Metabolomics technology and bioinformatics

338Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Shi, H., Vigneau-Callahan, K. E., Shestopalov, A. I., Milbury, P. E., Matson, W. R., & Kristal, B. S. (2002). Characterization of diet-dependent metabolic serotypes: Primary validation of male and female serotypes in independent cohorts of rats. Journal of Nutrition, 132(5), 1039–1046. https://doi.org/10.1093/jn/132.5.1039

Readers over time

‘10‘11‘12‘13‘14‘16‘19‘20‘22‘23‘24‘2502468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 10

53%

Researcher 5

26%

Professor / Associate Prof. 4

21%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 7

44%

Medicine and Dentistry 6

38%

Environmental Science 2

13%

Chemistry 1

6%

Save time finding and organizing research with Mendeley

Sign up for free
0