Development of Spectrophotometric methods for Assay of Salbutamol in Pharmaceutical Formulations

Abstract

‫ﺍﻟﺨﻼﺼﺔ‬ ‫ﺘﻤﻴﺯﺘﺎ‬ ‫ﻁﻴﻔﻴﺘﺎﻥ‬ ‫ﻁﺭﻴﻘﺘﺎﻥ‬ ‫ﻭﺼﻔﺕ‬ ‫ﺒ‬ ‫ﺎﻟ‬ ‫ﻭﻓـﻲ‬ ‫ﺍﻟﻨﻘـﻲ‬ ‫ﺒﺸﻜﻠﻪ‬ ‫ﺍﻟﺴﺎﻟﺒﻴﻭﺘﺎﻤﻭل‬ ‫ﻟﺘﻘﺩﻴﺭ‬ ‫ﺤﺴﺎﺴﻴﺔ‬ ‫ﺍﻟﺼﻴﺩﻻﻨﻴﺔ‬ ‫ﻤﺴﺘﺤﻀﺭﺍﺘﻪ‬. ‫ﺃﻴـﻭﻥ‬ ‫ﺒﻭﺴـﺎﻁﺔ‬ ‫ﺍﻟـﺴﺎﻟﺒﻴﻭﺘﺎﻤﻭل‬ ‫ﺃﻜﺴﺩﺓ‬ ‫ﻋﻠﻰ‬ ‫ﺍﻟﻁﺭﻴﻘﺘﻴﻥ‬ ‫ﻜﻠﺘﺎ‬ ‫ﺍﻋﺘﻤﺩﺕ‬ ‫ﺤﺎﻤﻀﻲ‬ ‫ﻭﺴﻁ‬ ‫ﻓﻲ‬ ‫ﺍﻟﺜﻼﺜﻲ‬ ‫ﺍﻟﺤﺩﻴﺩ‬. ‫ﺍﻟﻁﺭﻴﻘﺔ‬ ‫ﻓﻲ‬ A ‫ﻤـﻊ‬ ‫ﺍﻟﻤﺘﺤـﺭﺭ‬ ‫ﺍﻟﺜﻨـﺎﺌﻲ‬ ‫ﺍﻟﺤﺩﻴـﺩ‬ ‫ﻤﻔﺎﻋﻠﺔ‬ ‫ﺘﻡ‬ ‫ﺍﻟﻜﺎﺸﻑ‬ 10,1- ‫ﺍﻟﻔﻴﺭ‬ ‫ﻤﻌﻘﺩ‬ ‫ﻭﻗﻴﺱ‬ ‫ﻓﻴﻨﺎﻨﺜﺭﻭﻟﻴﻥ‬ ‫ﻋﻨ‬ ‫ﺍﻟﻤﻠﻭﻥ‬ ‫ﻭﻴﻥ‬ ‫ﺃﻟﻤﻭﺠﻲ‬ ‫ﺍﻟﻁﻭل‬ ‫ﺩ‬ 510 ‫ﻨـﺎﻨﻭﻤﻴﺘﺭ‬ ‫ﺍﻟﻁﺭﻴﻘﺔ‬ ‫ﻓﻲ‬ ‫ﺃﻤﺎ‬ ،‫ﺍﻟﺼﻭﺭﻱ‬ ‫ﻤﺤﻠﻭﻟﻪ‬ ‫ﻤﻘﺎﺒل‬ B ‫ﺍﻟﻜﺎﺸـﻑ‬ ‫ﻤـﻊ‬ ‫ﻤﻔﺎﻋﻠﺘﻪ‬ ‫ﺘﻤﺕ‬ ‫ﻓﻘﺩ‬ 2،2 َ-‫ﺜﻨـﺎﺌﻲ‬ ‫ﺃﻟﻤﻭﺠﻲ‬ ‫ﺍﻟﻁﻭل‬ ‫ﻋﻨﺩ‬ ‫ﺍﻟﻤﻠﻭﻥ‬ ‫ﺍﻟﻤﻌﻘﺩ‬ ‫ﻭﻗﻴﺱ‬ ‫ﺍﻟﺒﺭﻴﺩﻴل‬ 522 ‫ﺍﻟﺼﻭﺭﻱ‬ ‫ﻤﺤﻠﻭﻟﻪ‬ ‫ﻤﻘﺎﺒل‬ ‫ﻨﺎﻨﻭﻤﻴﺘﺭ‬. ‫ﻟﻘﺩ‬ ‫ﺍﻟﺘﺭﺍﻜﻴﺯ‬ ‫ﻤﺩﻯ‬ ‫ﻀﻤﻥ‬ ‫ﺒﻴﺭ‬ ‫ﻗﺎﻨﻭﻥ‬ ‫ﺘﻁﺒﻴﻕ‬ ‫ﺃﻤﻜﻥ‬ 0.15-6.00 ‫ﻤﺎﻴﻜﺭﻭﻏﺭﺍﻡ‬ / ‫ﻭ‬ ‫ﻤﻠﻠﺘـﺭ‬ 0.25-8.00 ‫ﻤﺎﻴﻜﺭﻭﻏﺭﺍﻡ‬ / ‫ﻤﻭﻻﺭﻴﺔ‬ ‫ﻭﺒﺎﻤﺘﺼﺎﺼﻴﺎﺕ‬ ‫ﻤﻠﻠﺘﺭ‬ 7.858×10 4 ‫ﻭ‬ 4.555×10 4 ‫ﻟﺘﺭ‬ / ‫ﻤﻭل‬. ‫ﻓـﻲ‬ ‫ﺴﻡ‬ ‫ﺍﻟﺘﻭﺍﻟﻲ‬ ‫ﻋﻠﻰ‬ ‫ﻭﺍﻟﺜﺎﻨﻴﺔ‬ ‫ﺍﻷﻭﻟﻰ‬ ‫ﺍﻟﻁﺭﻴﻘﺘﻴﻥ‬. ‫ﺍﻻﺴـﺘﺭﺠﺎﻉ‬ ‫ﻨـﺴﺒﺔ‬ ‫ﻤﻌـﺩل‬ ‫ﻭﺠﺩ‬ ‫ﻜﻤﺎ‬ 100.39 % ‫ﻭ‬ 99.11 % ‫ﺍﻟﺘﻭﺍﻟﻲ‬ ‫ﻭﻋﻠﻰ‬ ‫ﺍﻟﻁﺭﻴﻘﺘﻴﻥ‬ ‫ﻟﻜﻠﺘﺎ‬. ‫ﻤﺴﺘﺤـﻀﺭﺍﺘﻪ‬ ‫ﻓـﻲ‬ ‫ﺍﻟـﺴﺎﻟﺒﻴﻭﺘﺎﻤﻭل‬ ‫ﺘﻘﺩﻴﺭ‬ ‫ﻨﺘﺎﺌﺞ‬ ‫ﺇﻥ‬ ‫ﺃﺜﺒ‬ ‫ﺍﻟﺼﻴﺩﻻﻨﻴﺔ‬ ‫ﺘـﻡ‬ ‫ﻭﻗـﺩ‬ ‫ﺍﻟﻤﺴﺘﺤـﻀﺭﺍﺕ‬ ‫ﺘﻠﻙ‬ ‫ﺇﻟﻰ‬ ‫ﺍﻟﻤﻀﺎﻓﺔ‬ ‫ﺍﻟﺴﻭﺍﻍ‬ ‫ﻟﻤﻭﺍﺩ‬ ‫ﺘﺩﺍﺨل‬ ‫ﻭﺠﻭﺩ‬ ‫ﻋﺩﻡ‬ ‫ﺘﺕ‬ ‫ﺃﺨﺭﻯ‬ ‫ﻁﺭﺍﺌﻕ‬ ‫ﻭﻤﻊ‬ ‫ﺍﻟﺒﺭﻴﻁﺎﻨﻲ‬ ‫ﺍﻟﺩﺴﺘﻭﺭ‬ ‫ﻓﻲ‬ ‫ﺍﻟﻤﻌﺘﻤﺩﺓ‬ ‫ﺍﻟﻘﻴﺎﺴﻴﺔ‬ ‫ﺍﻟﻁﺭﻴﻘﺔ‬ ‫ﻤﻊ‬ ‫ﺒﻨﺠﺎﺡ‬ ‫ﻤﻘﺎﺭﻨﺘﻬﺎ‬. Abstract Two sensitive spectrophotometric methods are proposed for the determination of salbutamol in pure form and pharmaceutical preparations. The methods are based on the oxidation of salbutamol drug with Fe(III) in acidic medium. The liberated Fe(II) reacts with 1,10-phenanthroline in method A and the ferroin complex is colorimetrically measured at 510 nm against reagent blank. Method B is based on the reaction of the liberated Fe(II) with 2,2'-bipyridyl to form a stable coloured complex with maximum absorption at 520 nm against reagent blank. Beer's law was obeyed in the concentration range of 0.15-6.00 µg/ml and 0.25-8.00µg/ml with molar absorptivity of 7.858×10 4 and