Dose-response effects of intrauminal infusion of propionate on feeding behavior of lactating cows in early or midlactation

Abstract

The objective of this experiment was to evaluate whether dose-response effects of intraruminal infusion of propionate on feeding behavior and dry matter intake (DMI) differ by stage of lactation. Six cows in early lactation (EL) and six cows in midlactation (ML) were assigned to blocks in a duplicated 6 x 6 Latin square design experiment. Treatments were mixtures of sodium propionate and sodium acetate containing sodium propionate at 0, 20, 40, 60, 80, and 100% of total volatile fatty acids (VFA), infused into the rumen continuously for 18 h starting 6 h before feeding at a rate of 21.7 mmol of sodium VFA/min. All cows were ruminally cannulated prior to the experiment. The diet was formulated to contain 30% NDF, and dry cracked corn was the major source of starch. We hypothesized that hypophagic effects of propionate infusion were greater for EL compared with ML because of greater plasma concentration of nonesterified fatty acids (275 vs. 76 μMeq/ L) and expected greater basal oxidative metabolism in the liver for EL compared to ML. Propionate infusion decreased DMI for EL and ML, but a quadratic effect of propionate infusion was observed for ML but not EL. This indicated a greater marginal reduction in DMI at higher doses of propionate for ML compared to EL, contrary to our hypothesis. Propionate infusion decreased meal size similarly for both stages of lactation, but linearly increased intermeal interval for ML but not EL. We speculate that lower milk yield for ML compared with EL (30.8 vs. 42.0 kg/d) decreased glucose demand by the mammary gland and increased the proportion of infused propionate oxidized in the liver for ML compared to EL.